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Biodesign International Inc
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Upstate Biotechnology Inc
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Image Search Results
Journal:
Article Title: Elevated Neuronal Expression of CD200 Protects Wld s Mice from Inflammation-Mediated Neurodegeneration
doi: 10.2353/ajpath.2007.060677
Figure Lengend Snippet: CD200R expression in Wlds and WT splenocytes and CNS. CD200R expression was assessed by flow cytometry in WT and Wlds splenocyte populations on day 0 (a) and at day 15 (b) after immunization. CD200R was predominantly found on naïve CD11b+ and CD11c+ cells in both strains with no significant difference between the WT and Wlds strains. Expression of CD200R decreased after immunization on these cells. c: Protein levels were assessed in spinal cord lysates from WT and Wlds mice on day 0 and day 15 after immunization, by Western blot, and expressed as protein/β-actin IDV values. There was no significant difference in CD200R expression between the strains. IL-6 was reduced and IL-10 was elevated in Wlds mice compared with WT mice after immunization at day 15 after immunization. There was no significant difference in tumor necrosis factor-α, TLR-4, or CD22 expression between the two strains at either time point. d: Fractalkine (CX3CL1) concentration in spinal cord homogenates from WT and Wlds at day 0 and day 14 after immunization was assessed using ELISA assay. Results from four mice/group/time point were averaged. There was no significant difference between WT and Wlds mice at either time point.
Article Snippet: Antibodies Used for Immunofluorescence Staining The following antibodies were used: CD200 (clone 3B6, isotype rat IgM, 1:200; Cedarlane Laboratories, Hornby, ON, Canada); secondary: Alexa 488-conjugated goat anti-rat IgM (Molecular Probes);
Techniques: Expressing, Flow Cytometry, Western Blot, Concentration Assay, Enzyme-linked Immunosorbent Assay
Journal:
Article Title: Elevated Neuronal Expression of CD200 Protects Wld s Mice from Inflammation-Mediated Neurodegeneration
doi: 10.2353/ajpath.2007.060677
Figure Lengend Snippet: CD200R is expressed on microglia, oligodendrocytes, and astrocytes. Spinal cord sections from WT and Wlds mice on days 0, 22, and 60 after immunization were double-stained with CD200R (red) and LB4-microglial or GFAP (astrocytes) or CNPase (oligodendrocytes) or β-tubulin (axons and neurons). Shown are representative merged confocal images from mice on day 22 after immunization. CD200R staining was present on microglia/macrophages as well as astrocytes and oligodendrocytes from both strains. CD200R was not present on axons/neurons. Original magnifications, ×63.
Article Snippet: Antibodies Used for Immunofluorescence Staining The following antibodies were used: CD200 (clone 3B6, isotype rat IgM, 1:200; Cedarlane Laboratories, Hornby, ON, Canada); secondary: Alexa 488-conjugated goat anti-rat IgM (Molecular Probes);
Techniques: Staining
Journal: Oncology Letters
Article Title: Coexistence of adenomyosis uteri and endometrial cancer is associated with an improved prognosis compared with endometrial cancer only
doi: 10.3892/ol.2017.6592
Figure Lengend Snippet: Primary antibodies used in the study.
Article Snippet:
Techniques:
Journal: Oncology Letters
Article Title: Coexistence of adenomyosis uteri and endometrial cancer is associated with an improved prognosis compared with endometrial cancer only
doi: 10.3892/ol.2017.6592
Figure Lengend Snippet: Expression of the different proteins in the different groups.
Article Snippet:
Techniques: Expressing
Journal: Oncology Letters
Article Title: Coexistence of adenomyosis uteri and endometrial cancer is associated with an improved prognosis compared with endometrial cancer only
doi: 10.3892/ol.2017.6592
Figure Lengend Snippet: Glycodelin expression in Group A (patients with endometrioid endometrial cancer and adenomyosis uteri). Representative microphotographs of glycodelin immunohistochemical staining are shown (immunoreactivity score, 4).
Article Snippet:
Techniques: Expressing, Immunohistochemical staining, Staining
Journal: Oncology Letters
Article Title: Coexistence of adenomyosis uteri and endometrial cancer is associated with an improved prognosis compared with endometrial cancer only
doi: 10.3892/ol.2017.6592
Figure Lengend Snippet: Glycodelin expression in Group B (patients with endometrioid endometrial cancer only). Representative microphotographs of glycodelin immunohistochemical staining are shown (immunoreactivity score, 6).
Article Snippet:
Techniques: Expressing, Immunohistochemical staining, Staining