mouse igg1κ antibody Search Results


90
Trillium Therapeutics cd200r antibody (anti-313015 cd200r peptide, 26 clone r252)
<t>CD200R</t> expression in Wlds and WT splenocytes and CNS. CD200R expression was assessed by flow cytometry in WT and Wlds splenocyte populations on day 0 (a) and at day 15 (b) after immunization. CD200R was predominantly found on naïve CD11b+ and CD11c+ cells in both strains with no significant difference between the WT and Wlds strains. Expression of CD200R decreased after immunization on these cells. c: Protein levels were assessed in spinal cord lysates from WT and Wlds mice on day 0 and day 15 after immunization, by Western blot, and expressed as protein/β-actin IDV values. There was no significant difference in CD200R expression between the strains. IL-6 was reduced and IL-10 was elevated in Wlds mice compared with WT mice after immunization at day 15 after immunization. There was no significant difference in tumor necrosis factor-α, TLR-4, or CD22 expression between the two strains at either time point. d: Fractalkine (CX3CL1) concentration in spinal cord homogenates from WT and Wlds at day 0 and day 14 after immunization was assessed using ELISA assay. Results from four mice/group/time point were averaged. There was no significant difference between WT and Wlds mice at either time point.
Cd200r Antibody (Anti 313015 Cd200r Peptide, 26 Clone R252), supplied by Trillium Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Zytomed Inc glycodelin mouse (igg1κ) 6f2 antibody
Primary antibodies used in the study.
Glycodelin Mouse (Igg1κ) 6f2 Antibody, supplied by Zytomed Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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GenScript corporation mouse monoclonal tau antibody 8b2 igg1κ
Primary antibodies used in the study.
Mouse Monoclonal Tau Antibody 8b2 Igg1κ, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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NanoView Biosciences mouse igg1κ matching isotype antibody
Primary antibodies used in the study.
Mouse Igg1κ Matching Isotype Antibody, supplied by NanoView Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biodesign International Inc horseradish peroxidase-conjugated rat igg1κ that binds mouse igg κ light chain (clone lo-mk-2) antibody
Primary antibodies used in the study.
Horseradish Peroxidase Conjugated Rat Igg1κ That Binds Mouse Igg κ Light Chain (Clone Lo Mk 2) Antibody, supplied by Biodesign International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Upstate Biotechnology Inc unconjugated mouse anti-human c-met monoclonal antibody (moab) (igg1κ)
Primary antibodies used in the study.
Unconjugated Mouse Anti Human C Met Monoclonal Antibody (Moab) (Igg1κ), supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck & Co isotype control mouse igg1-κ antibody
Primary antibodies used in the study.
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Abnova mouse monoclonal igg 1κ primary antibody
Primary antibodies used in the study.
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Nichirei Corporation histofine cd8 mouse igg1κ mab antibody
Primary antibodies used in the study.
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Image Search Results


CD200R expression in Wlds and WT splenocytes and CNS. CD200R expression was assessed by flow cytometry in WT and Wlds splenocyte populations on day 0 (a) and at day 15 (b) after immunization. CD200R was predominantly found on naïve CD11b+ and CD11c+ cells in both strains with no significant difference between the WT and Wlds strains. Expression of CD200R decreased after immunization on these cells. c: Protein levels were assessed in spinal cord lysates from WT and Wlds mice on day 0 and day 15 after immunization, by Western blot, and expressed as protein/β-actin IDV values. There was no significant difference in CD200R expression between the strains. IL-6 was reduced and IL-10 was elevated in Wlds mice compared with WT mice after immunization at day 15 after immunization. There was no significant difference in tumor necrosis factor-α, TLR-4, or CD22 expression between the two strains at either time point. d: Fractalkine (CX3CL1) concentration in spinal cord homogenates from WT and Wlds at day 0 and day 14 after immunization was assessed using ELISA assay. Results from four mice/group/time point were averaged. There was no significant difference between WT and Wlds mice at either time point.

Journal:

Article Title: Elevated Neuronal Expression of CD200 Protects Wld s Mice from Inflammation-Mediated Neurodegeneration

doi: 10.2353/ajpath.2007.060677

Figure Lengend Snippet: CD200R expression in Wlds and WT splenocytes and CNS. CD200R expression was assessed by flow cytometry in WT and Wlds splenocyte populations on day 0 (a) and at day 15 (b) after immunization. CD200R was predominantly found on naïve CD11b+ and CD11c+ cells in both strains with no significant difference between the WT and Wlds strains. Expression of CD200R decreased after immunization on these cells. c: Protein levels were assessed in spinal cord lysates from WT and Wlds mice on day 0 and day 15 after immunization, by Western blot, and expressed as protein/β-actin IDV values. There was no significant difference in CD200R expression between the strains. IL-6 was reduced and IL-10 was elevated in Wlds mice compared with WT mice after immunization at day 15 after immunization. There was no significant difference in tumor necrosis factor-α, TLR-4, or CD22 expression between the two strains at either time point. d: Fractalkine (CX3CL1) concentration in spinal cord homogenates from WT and Wlds at day 0 and day 14 after immunization was assessed using ELISA assay. Results from four mice/group/time point were averaged. There was no significant difference between WT and Wlds mice at either time point.

Article Snippet: Antibodies Used for Immunofluorescence Staining The following antibodies were used: CD200 (clone 3B6, isotype rat IgM, 1:200; Cedarlane Laboratories, Hornby, ON, Canada); secondary: Alexa 488-conjugated goat anti-rat IgM (Molecular Probes); CD200R (anti-313015 CD200R peptide, 26 clone R252, isotype rabbit IgG, 1:200; Trillium Therapeutics Inc.); secondary: Alexa 594-conjugated goat anti-rabbit IgG (Molecular Probes); CD200R (clone OX-110, isotype rat IgG2a, 1:100; Serotec, Oxford, UK); secondary: Alexa 488-conjugated rabbit anti-rat IgG (Molecular Probes); mitogen-activated protein 2 (MAP-2) (clone HM-2: mouse anti-mouse IgG1, 1:100; Sigma); secondary: Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); NeuN (clone A60, isotype mouse IgG1, 1:100; Chemicon/Millipore, Temecula, CA); secondary: Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); GFAP cocktail (clones 4A11, 1B4, 2E1, isotype mouse IgG2b, 1:100; BD Pharmingen, Palo Alto, CA); secondary: Alexa 488- or Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); CNPase (clone 11-5B, isotype mouse IgG1, 1:100; Chemicon/Millipore); secondary: Alexa 488- or Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); β-tubulin (clone TUJ1, isotype mouse IgG2a; 1:100; Covance, Berkeley, CA); secondary: Alexa 488- or Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); CD4 (clone H29.129; isotype rat IgG2a; 1:50; BD Pharmingen); secondary: Alexa-488-conjugated goat anti-rat IgG (Molecular Probes); and CD8 (clone 53-6.7; isotype rat IgG2a, 1:50; BD Pharmingen); secondary: Alexa-488-conjugated goat anti-rat IgG (Molecular Probes).

Techniques: Expressing, Flow Cytometry, Western Blot, Concentration Assay, Enzyme-linked Immunosorbent Assay

CD200R is expressed on microglia, oligodendrocytes, and astrocytes. Spinal cord sections from WT and Wlds mice on days 0, 22, and 60 after immunization were double-stained with CD200R (red) and LB4-microglial or GFAP (astrocytes) or CNPase (oligodendrocytes) or β-tubulin (axons and neurons). Shown are representative merged confocal images from mice on day 22 after immunization. CD200R staining was present on microglia/macrophages as well as astrocytes and oligodendrocytes from both strains. CD200R was not present on axons/neurons. Original magnifications, ×63.

Journal:

Article Title: Elevated Neuronal Expression of CD200 Protects Wld s Mice from Inflammation-Mediated Neurodegeneration

doi: 10.2353/ajpath.2007.060677

Figure Lengend Snippet: CD200R is expressed on microglia, oligodendrocytes, and astrocytes. Spinal cord sections from WT and Wlds mice on days 0, 22, and 60 after immunization were double-stained with CD200R (red) and LB4-microglial or GFAP (astrocytes) or CNPase (oligodendrocytes) or β-tubulin (axons and neurons). Shown are representative merged confocal images from mice on day 22 after immunization. CD200R staining was present on microglia/macrophages as well as astrocytes and oligodendrocytes from both strains. CD200R was not present on axons/neurons. Original magnifications, ×63.

Article Snippet: Antibodies Used for Immunofluorescence Staining The following antibodies were used: CD200 (clone 3B6, isotype rat IgM, 1:200; Cedarlane Laboratories, Hornby, ON, Canada); secondary: Alexa 488-conjugated goat anti-rat IgM (Molecular Probes); CD200R (anti-313015 CD200R peptide, 26 clone R252, isotype rabbit IgG, 1:200; Trillium Therapeutics Inc.); secondary: Alexa 594-conjugated goat anti-rabbit IgG (Molecular Probes); CD200R (clone OX-110, isotype rat IgG2a, 1:100; Serotec, Oxford, UK); secondary: Alexa 488-conjugated rabbit anti-rat IgG (Molecular Probes); mitogen-activated protein 2 (MAP-2) (clone HM-2: mouse anti-mouse IgG1, 1:100; Sigma); secondary: Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); NeuN (clone A60, isotype mouse IgG1, 1:100; Chemicon/Millipore, Temecula, CA); secondary: Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); GFAP cocktail (clones 4A11, 1B4, 2E1, isotype mouse IgG2b, 1:100; BD Pharmingen, Palo Alto, CA); secondary: Alexa 488- or Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); CNPase (clone 11-5B, isotype mouse IgG1, 1:100; Chemicon/Millipore); secondary: Alexa 488- or Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); β-tubulin (clone TUJ1, isotype mouse IgG2a; 1:100; Covance, Berkeley, CA); secondary: Alexa 488- or Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); CD4 (clone H29.129; isotype rat IgG2a; 1:50; BD Pharmingen); secondary: Alexa-488-conjugated goat anti-rat IgG (Molecular Probes); and CD8 (clone 53-6.7; isotype rat IgG2a, 1:50; BD Pharmingen); secondary: Alexa-488-conjugated goat anti-rat IgG (Molecular Probes).

Techniques: Staining

Primary antibodies used in the study.

Journal: Oncology Letters

Article Title: Coexistence of adenomyosis uteri and endometrial cancer is associated with an improved prognosis compared with endometrial cancer only

doi: 10.3892/ol.2017.6592

Figure Lengend Snippet: Primary antibodies used in the study.

Article Snippet: Glycodelin , Mouse (IgG1κ) , 6F2 , 116–0646 , Zytomed Systems GmbH, Berlin, Germany , 1:4,000 in PBS.

Techniques:

Expression of the different proteins in the different groups.

Journal: Oncology Letters

Article Title: Coexistence of adenomyosis uteri and endometrial cancer is associated with an improved prognosis compared with endometrial cancer only

doi: 10.3892/ol.2017.6592

Figure Lengend Snippet: Expression of the different proteins in the different groups.

Article Snippet: Glycodelin , Mouse (IgG1κ) , 6F2 , 116–0646 , Zytomed Systems GmbH, Berlin, Germany , 1:4,000 in PBS.

Techniques: Expressing

Glycodelin expression in Group A (patients with endometrioid endometrial cancer and adenomyosis uteri). Representative microphotographs of glycodelin immunohistochemical staining are shown (immunoreactivity score, 4).

Journal: Oncology Letters

Article Title: Coexistence of adenomyosis uteri and endometrial cancer is associated with an improved prognosis compared with endometrial cancer only

doi: 10.3892/ol.2017.6592

Figure Lengend Snippet: Glycodelin expression in Group A (patients with endometrioid endometrial cancer and adenomyosis uteri). Representative microphotographs of glycodelin immunohistochemical staining are shown (immunoreactivity score, 4).

Article Snippet: Glycodelin , Mouse (IgG1κ) , 6F2 , 116–0646 , Zytomed Systems GmbH, Berlin, Germany , 1:4,000 in PBS.

Techniques: Expressing, Immunohistochemical staining, Staining

Glycodelin expression in Group B (patients with endometrioid endometrial cancer only). Representative microphotographs of glycodelin immunohistochemical staining are shown (immunoreactivity score, 6).

Journal: Oncology Letters

Article Title: Coexistence of adenomyosis uteri and endometrial cancer is associated with an improved prognosis compared with endometrial cancer only

doi: 10.3892/ol.2017.6592

Figure Lengend Snippet: Glycodelin expression in Group B (patients with endometrioid endometrial cancer only). Representative microphotographs of glycodelin immunohistochemical staining are shown (immunoreactivity score, 6).

Article Snippet: Glycodelin , Mouse (IgG1κ) , 6F2 , 116–0646 , Zytomed Systems GmbH, Berlin, Germany , 1:4,000 in PBS.

Techniques: Expressing, Immunohistochemical staining, Staining